AquaVerify

Plaque assay

A plaque assay is a culture method that quantifies bacteriophages through visible lysis plaques formed on a bacterial lawn.

Methods and sampling

Definition

A plaque assay is a culture method that quantifies bacteriophages through visible lysis plaques formed on a bacterial lawn.

Useful for selecting the right method, product family, sampling point, report or traceability workflow in water quality programs.

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Method blank A method blank is a control that accompanies the analytical process without the target sample to detect contamination introduced during preparation, reagents or handling.
Cold chain Cold chain is the controlled-temperature condition maintained during transport and storage of a sample or sensitive material.
Negative control A negative control is a material or condition that should not produce a signal and helps detect contamination, false positives or handling errors.
Positive control A positive control is a material or condition that should produce the expected response and confirms that the method can detect the target.
Double Agar Layer Double Agar Layer is a classic two-layer agar procedure used in bacteriophage assays, where an upper layer containing sample and host is poured over an agar base.
Enrichment Enrichment is an analytical step that promotes growth or multiplication of a target microorganism before detection.
Membrane filtration Membrane filtration is a technique in which a volume of water is filtered and the membrane is incubated on a medium to recover and count microorganisms.
Incubation Incubation is maintaining a sample or culture under controlled temperature and time to allow microbiological growth, reaction or reading.

A plaque assay is a culture method that quantifies bacteriophages through visible lysis plaques formed on a bacterial lawn.

Useful for selecting the right method, product family, sampling point, report or traceability workflow in water quality programs.