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Limit of detection (LOD)

The limit of detection is the lowest level of analyte or microorganism that a method can detect under defined conditions, without necessarily implying exact quantification.

Limit of detection (LOD)

Methods and sampling

Definition

The limit of detection is the lowest level of analyte or microorganism that a method can detect under defined conditions, without necessarily implying exact quantification.

Why it matters

Useful for selecting the right method, product family, sampling point, report or traceability workflow in water quality programs.

  • Related AquaVerify product: Kits ISO/EPA / AquaVerify Cloud
  • Related sector: Laboratorios y OEM

Related resources

Explore technical guides, AquaVerify products, sector applications and authorized support connected with this definition.

Related terms

  • Method blank A method blank is a control that accompanies the analytical process without the target sample to detect contamination introduced during preparation, reagents or handling.
  • Cold chain Cold chain is the controlled-temperature condition maintained during transport and storage of a sample or sensitive material.
  • Negative control A negative control is a material or condition that should not produce a signal and helps detect contamination, false positives or handling errors.
  • Positive control A positive control is a material or condition that should produce the expected response and confirms that the method can detect the target.
  • Double Agar Layer Double Agar Layer is a classic two-layer agar procedure used in bacteriophage assays, where an upper layer containing sample and host is poured over an agar base.
  • Enrichment Enrichment is an analytical step that promotes growth or multiplication of a target microorganism before detection.
  • Plaque assay A plaque assay is a culture method that quantifies bacteriophages through visible lysis plaques formed on a bacterial lawn.
  • Membrane filtration Membrane filtration is a technique in which a volume of water is filtered and the membrane is incubated on a medium to recover and count microorganisms.

FAQ

Definition: Limit of detection (LOD)

The limit of detection is the lowest level of analyte or microorganism that a method can detect under defined conditions, without necessarily implying exact quantification.

Why it matters: Limit of detection (LOD)

Useful for selecting the right method, product family, sampling point, report or traceability workflow in water quality programs.